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KMID : 0616120190500010011
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Korean Journal of Pharmacognosy
2019 Volume.50 No. 1 p.11 ~ p.17
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Anti-inflammatory Effects of Aurantio-obtusin isolated from Cassia tora L. in RAW264.7 Cells
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Lee Ki-Ho
Jang Ji-Hun
Woo Kyeong-Wan
Nho Jong-Hyun
Jung Ho-Kyung
Cho Hyun-Woo
Yong Ju-Hyun
An Byeong-Kwan
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Abstract
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Cassia tora L. have been used as a folk medicine in Korea. This study investigated anti-inflammatory effect of aurantio-obtusin isolated from C. tora. We isolated aurantio-obtusin from 50% ethanol extracts of C. tora L. We investigated the anti-inflammatory effects of aurantio-obtusin on the lipopolysaccharide (LPS)-stimulated inflammatory response in murine macrophage cell line (Raw 264.7). To investigate the cytotoxicity of aurantio-obtusin on RAW 264.7 cells, MTS assay was performed. RAW 264.7 cells were treated with aurantio-obtusin at different concentrations (12.5, 25, 50, 100 μM) for 30 h. The result showed that aurantio-obtusin had no cytotoxic effect in a concentration range of 12.5 ? 100 μM. To determine the effect of aurantio-obtusin on LPS-induced NO production, the NO concentration measurement was performed. RAW 264.7 cells were treated with aurantio-obtusin at 12.5, 25, 50 and 100 μM for 24 h, and the results showed that the NO production of aurantio-obtusin-treated cells compared to LPS alone treated group was significantly decreased in a dose-dependent manner. Pretreatment of aurantio-obtusin inhibited LPS-induced NO production in a dose-dependent manner. To find out inhibitory mechanisms of aurantio-obtusin on inflammatory mediators, we examined the PGE2 pathways. As a result, PGE2 were decreased in a dose-dependent manner by aurantio-obtusin. The release of interleukin-1b (IL-1b) and IL-6 were also reduced. Moreover, aurantio-obtusin suppressed LPL-induced IκB-a degradation. These results suggest that the down regulation of NO, PGE2, IL-1b and IL-6 expression by aurantio-obtusin are achieved by the downregulation of NF-κB activity.
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KEYWORD
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Cassia tora L., Aurantio-obtusin, RAW 264.7, Anti-inflammatory effect
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